LIF Antibody

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P15018

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 72-101 from the human protein was used as the immunogen for this LIF antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the LIF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This LIF antibody is available for research use only.

Storage Conditions

Aliquot the LIF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the LIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of 1) human CEM, 2) rat PC-12, and 3) mouse spleen tissue lysate using LIF antibody at 1:1000. Observed molecular weight ~23/32~62kDa (unmodified/glycosylated) .