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PAX6 Antibody

PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P26367

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 183-210 from the human protein was used as the immunogen for this PAX6 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IHC, IF, FACS, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the PAX6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PAX6 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the PAX6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the PAX6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Bovine, Xenopus

Location

Nuclear, cytoplasmic

Image Legend

PAX6 antibody western blot analysis in U251 lysate. Predicted molecular weight ~48kDa.

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