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VCP Antibody

VCP is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

P55072

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 726-755 from the human protein was used as the immunogen for this VCP antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the VCP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This VCP antibody is available for research use only.

Storage Conditions

Aliquot the VCP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the VCP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Pig, Rat, Xenopus, Zebrafish

Image Legend

Western blot analysis of VCP antibody and 1) Jurkat, 2) 293, and 3) MDA-MB231 lysate. Predicted/observed molecular weight: ~89/97kDa.

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