VCP Antibody
VCP is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P55072
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 726-755 from the human protein was used as the immunogen for this VCP antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the VCP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This VCP antibody is available for research use only.
Storage Conditions
Aliquot the VCP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the VCP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Mouse, Pig, Rat, Xenopus, Zebrafish
Image Legend
Western blot analysis of VCP antibody and 1) Jurkat, 2) 293, and 3) MDA-MB231 lysate. Predicted/observed molecular weight: ~89/97kDa.
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