P65 Antibody (RELA)
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
Q04206
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 10-37 from the human protein was used as the immunogen for this p65 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, FACS, IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the p65 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This p65 antibody is available for research use only.
Storage Conditions
Aliquot the p65 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Chicken, Mouse, Xenopus
Image Legend
P65 antibody western blot analysis in (1) HeLa, (2) MCF-7, (3) Raji and (4) Ramos lysate.
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Frequently Asked Questions
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