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P65 Antibody (RELA)

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

Q04206

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 10-37 from the human protein was used as the immunogen for this p65 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IHC, FACS, IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the p65 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This p65 antibody is available for research use only.

Storage Conditions

Aliquot the p65 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Chicken, Mouse, Xenopus

Image Legend

P65 antibody western blot analysis in (1) HeLa, (2) MCF-7, (3) Raji and (4) Ramos lysate.

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