VDAC Antibody
VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P21796
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 1-30 from human VDAC1 was used as the immunogen for this VDAC antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the VDAC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This VDAC antibody is available for research use only.
Storage Conditions
Aliquot the VDAC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the VDAC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Pig, Rabbit, Rat
Image Legend
Western blot analysis of VDAC antibody and 1) human A375 cell line and 2) mouse heart tissue lysate. Predicted molecular weight 30~35 kDa.
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