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C-Fos Antibody

The Fos family consists of 4 members: c-FOS, FOSB, FOSL1, and FOSL2. These leucine zipper proteins can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, these proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the c-Fos gene has also been associated with apoptotic cell death.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

P01100

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 263-291 from the human protein was used as the immunogen for this c-Fos antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the c-Fos antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This c-Fos antibody is available for research use only.

Storage Conditions

Aliquot the c-Fos antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the c-Fos antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rat, Bovine

Image Legend

Western blot analysis of c-Fos antibody and 293 lysate. Expected molecular weight: ~40 kDa (unmodified), 53-68 kDa (phosphorylated) .

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