TRAP Antibody / CD40L

TRAP/CD154/CD40L is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P29965

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 33-62 from the human protein was used as the immunogen for this TRAP antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, IF, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TRAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TRAP antibody is available for research use only.

Storage Conditions

Aliquot the TRAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the TRAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Pig

Location

Cytoplasmic, membranous

Image Legend

Western blot analysis of TRAP antibody and NCI-H460 lysate. Expected molecular weight: 29-39 kDa (depending on glycosylation level) or ~18 kDa (soluble form) .