G-Actin Antibody / Gamma Actin / ACTG1
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Flow cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
P63261
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 188-215 from human ACTG1 was used as the immunogen for this g-Actin antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the g-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This g-Actin antibody is available for research use only.
Storage Conditions
Aliquot the g-Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the g-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, C. elegans, Chicken, Drosophila, Mouse, Rabbit, Rat, Xenopus, Zebrafish
Image Legend
Western blot analysis of g-Actin antibody and (1) K562 and (2) HepG2 lysate.
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