Parkin Antibody / PARK2

Parkin possibly plays a role in the regulation of neuron death. Limits the production of reactive oxygen species (ROS) . Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress. Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50, IHC (Paraffin) : 1:50-1:100

UniProt

O60260

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 387-417 from the human protein was used as the immunogen for this Parkin antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, FACS, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Parkin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Parkin antibody is available for research use only.

Storage Conditions

Aliquot the Parkin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Parkin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Confocal immunofluorescent analysis of Parkin antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) . DAPI was used as a nuclear counterstain (blue) .