NUR77 Antibody

NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. This protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P22736

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 329-358 from human NR4A1 was used as the immunogen for this NUR77 antibody. (1)

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NUR77 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NUR77 antibody is available for research use only.

Storage Conditions

Aliquot the NUR77 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the NUR77 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. This Ab will detect isoforms 1 and 2.

Prediction Reactivity

Mouse, Rat

Image Legend

Western blot analysis of NUR77 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the NR4A1 gene (2) . Predicted molecular weight ~64/66kDa (isoforms 1/2) .