GRB2 Antibody

GRB2 is an adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P62993

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 89-118 from the human protein was used as the immunogen for this GRB2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the GRB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GRB2 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the GRB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the GRB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Xenopus

Image Legend

Western blot analysis of GRB2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (2) . Predicted molecular weight ~25kDa.