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HES1 Antibody

HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. HES1 has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

Q14469

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 9-38 from the human protein was used as the immunogen for this HES1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, WB, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HES1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HES1 antibody is available for research use only.

Storage Conditions

Aliquot the HES1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the HES1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Rat, Xenopus

Image Legend

HES1 antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control (left histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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