Cdc25A Antibody

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

P30304

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 271-300 from the human protein was used as the immunogen for this Cdc25A antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the Cdc25A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Cdc25A antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the Cdc25A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Cdc25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.