SIRT3 Antibody

SIRT3 is an NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

Q8R104

Host

Rabbit

Reactivity

Human, Mouse, Hamster

Immunogen

A portion of amino acids 304-334 from the mouse protein was used as the immunogen for this SIRT3 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the SIRT3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SIRT3 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the SIRT3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SIRT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.