NOTCH3 Antibody

NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:50-1:100

UniProt

Q9UM47

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 2291-2321 from the human protein was used as the immunogen for this NOTCH3 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NOTCH3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NOTCH3 antibody is available for research use only.

Storage Conditions

Aliquot the NOTCH3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the NOTCH3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Confocal immunofluorescent analysis of NOTCH3 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .