Leptin Receptor Antibody / LEPR / Ob-R

Leptin, an adipocyte-specific hormone, regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure by acting through the leptin receptor (LEPR) . LEPR is a single-transmembrane-domain receptor of the cytokine receptor family that is identical to the mouse diabetes (db) gene product. During weight loss, leptin levels decrease, whereas soluble LEPR levels and the receptor bound fraction of leptin increases. The presence of LEPR in the absorptive cells of the small intestine suggests that leptin may have a physiological role in the regulation of nutrient absorption.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50, IHC (Paraffin) : 1:50-1:100

UniProt

P48357

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 23-52 from the human protein was used as the immunogen for this Leptin Receptor antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Leptin Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Leptin Receptor antibody is available for research use only.

Storage Conditions

Aliquot the Leptin Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Leptin Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Leptin Receptor antibody flow cytometric analysis of K562 cells (green) compared to a negative control (blue) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.