CUX1 Antibody / Homeobox protein cut-like 1

CUX1 likely has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:2000, IHC (Paraffin) : 1:25-1:50, Immunofluorescence: 1:10-1:50

UniProt

P39880

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1347-1374 from the human protein was used as the immunogen for this CUX1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CUX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CUX1 antibody is available for research use only.

Storage Conditions

Aliquot the CUX1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CUX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent confocal image of human HeLa cell stained with CUX1 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC) . Green = CUX1 antibody, Red = Phalloidin stained actin.