PPARG Antibody / PPAR gamma

PPARG is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

P37231

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this PPARG antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PPARG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PPARG antibody is available for research use only.

Storage Conditions

Aliquot the PPARG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PPARG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

PPARG antibody flow cytometric analysis of human HepG2 cells (green) compared to a negative control (blue) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.