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Beta III Tubulin Antibody / TUBB3

Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

Q13509

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 36-63 from the human protein was used as the immunogen for this beta III Tubulin antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the beta III Tubulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This beta III Tubulin antibody is available for research use only.

Storage Conditions

Aliquot the beta III Tubulin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the beta III Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Chicken, Rat

Image Legend

Beta III Tubulin antibody western blot analysis in HepG2 lysate

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