WNT5A Antibody
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P41221
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 185-213 from the human protein was used as the immunogen for this WNT5A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, FACS, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the WNT5A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This WNT5A antibody is available for research use only.
Storage Conditions
Aliquot the WNT5A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the WNT5A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Rabbit
Image Legend
IHC staining of FFPE human thyroid carcinoma with WNT5A antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.
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