WNT5A Antibody

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

P41221

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 185-213 from the human protein was used as the immunogen for this WNT5A antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, FACS, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the WNT5A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This WNT5A antibody is available for research use only.

Storage Conditions

Aliquot the WNT5A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the WNT5A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Rabbit

Image Legend

IHC staining of FFPE human thyroid carcinoma with WNT5A antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.