H4 Antibody / Histone H4
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P62805
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for this H4 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the H4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This H4 antibody is available for research use only.
Storage Conditions
Aliquot the H4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
H4 antibody western blot analysis in (1) human K562 and (2) mouse NIH3T3 lysate.
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