CD63 Antibody / LAMP-3

CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (FFPE) : 1:25, FACS: 1:25 per 1x10^6 cells

UniProt

P08962

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 163-190 from the human protein was used as the immunogen for this CD63 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD63 antibody is available for research use only.

Storage Conditions

Aliquot the CD63 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CD63 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasm, cell surface and secreted

Image Legend

Western blot testing of CD63 antibody at 1:2000 dilution. Lane 1: A2058 lysate; 2: A375 lysate; 3: THP-1 lysate; 4: U-87 MG lysate; Predicted molecular weight: 25-60 kDa depending on glycosylation level.