IL-4 Antibody

IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P05112

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 122-150 from the human protein was used as the immunogen for this IL-4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the IL-4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IL-4 antibody is available for research use only.

Storage Conditions

Aliquot the IL-4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Primate

Location

Secreted

Image Legend

(Left) Western blot analysis of IL-4 antibody and WiDr lysate; (Right) Western blot analysis of IL-4 antibody and mouse cerebellum lysate. Expected molecular weight: 14-20 kDa depending on glycosylation level.