XBP1 Antibody
XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1) . The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1 (S), which is the functionally active transcription factor.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P17861
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 62-91 from the human protein was used as the immunogen for this XBP1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the XBP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This XBP1 antibody is available for research use only.
Storage Conditions
Aliquot the XBP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the XBP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Bovine
Image Legend
Western blot analysis of XBP1 antibody and Ramos lysate. Predicted molecular weight: ~29 kDa (isoform 1) and ~40 kDa (isoform 2) .
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