HIF1 alpha Antibody
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) . This protein encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
Q16665
Host
Rabbit
Reactivity
Human, Hamster
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for this HIF1 alpha antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the HIF1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HIF1 alpha antibody is available for research use only.
Storage Conditions
Aliquot the HIF1 alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Location
Nuclear, possible cytoplasmic
Image Legend
Western blot analysis of HIF1 alpha antibody and CHO lysate. Routinely observed molecular weight: 100~120 kDa.
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