Phospho-Ikkb Antibody (pS701)
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) .
Product Specifications
CAS Number
9007-83-4
Specifications
Dot blot: 1:500
UniProt
O88351
Host
Rabbit
Reactivity
Mouse
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
DB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-IKKB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-Ikkb antibody is available for research use only.
Storage Conditions
Aliquot the phospho-Ikkb antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-Ikkb antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Dot blot analysis of phospho-Ikkbantibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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