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Phospho-ULK1 Antibody (pS556)

Involved in autophagy. Required for autophagosome formation (By similarity) . Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity) . Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity) . Plays an essential role in neurite extension of cerebellar granule cells (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:200, Dot blot: 1:500

UniProt

O75385

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-ULK1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS556 of human ULK1.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-ULK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-ULK1 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-ULK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/phospho-ulk1-antibody-ps556-f48759

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-ULK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent image of Chloroquine treated U251 cells stained with phospho-ULK1 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
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