Phospho-Bcl-2 Antibody (pS70)

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500, Western blot: 1:1000

UniProt

P10415

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-Bcl-2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS70 of human Bcl-2.

Clonality

Polyclonal

Isotype

Applications

WB, DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-Bcl-2 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-Bcl-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-Bcl-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of extracts from Jurkat cells, untreated or treated with Paclitaxel, using phospho Bcl-2 antibody (left) or nonphos Ab (right) .