Biotinylated Human HLA-A*24:02&B2M&p53 (TYSPALNKMF) Complex Protein (Monomer, MALS verified)

TP53 has been recognized as a tumor suppressor. 50% of cancers carry a TP53 mutation while many others affect other pathway components. High-copy numbers of WT p53 peptide-MHC class I complexes were detected on tumor cells as compared to low copies on normal cells. The Human HLA-A*0201 p53 (GLAPPQHLIRV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and GLAPPQHLIRV peptide of the p53.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*24:02&B2M&p53 (TYSPALNKMF) Complex Protein is produced by co-expression of HLA and B2M loaded with p53 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 35.8 kDa and 11.7 kDa. The protein migrates as 39-42 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59600.1 (HLA-A*24:02) & P61769 (B2M) TYSPALNKMF

Expression Region

Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & TYSPALNKMF peptide

Host

HEK293

Target

HLA-A*2402 & B2M & p53 (TYSPALNKMF)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human HLA-A*24:02&B2M&p53 (TYSPALNKMF) Complex Protein (Cat. No. HLP-H82Eb) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-A24&p53 Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (QC tested).