Phospho-Nephrin Antibody (pY1193)
Nephrin a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. It primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine.
Product Specifications
CAS Number
9007-83-4
Specifications
Dot blot: 1:500
UniProt
O60500
Host
Rabbit
Reactivity
Human
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
DB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-Nephrin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-Nephrin antibody is available for research use only.
Storage Conditions
Aliquot the phospho-Nephrin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-Nephrin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Dot blot analysis of phospho-Nephrin antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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