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Phospho-Nephrin Antibody (pY1193)

Nephrin a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. It primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500

UniProt

O60500

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-Nephrin antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1193 of human Nephrin.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-Nephrin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-Nephrin antibody is available for research use only.

Storage Conditions

Aliquot the phospho-Nephrin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-Nephrin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Dot blot analysis of phospho-Nephrin antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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