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Phosphorylated-SOX2 Antibody (pS251)

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT) .

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500

UniProt

P48431

Host

Rabbit

Reactivity

Human

Immunogen

This phosphorylated-SOX2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS251 of human SOX2.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the phosphorylated-SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phosphorylated-SOX2 antibody is available for research use only.

Storage Conditions

Aliquot the phosphorylated-SOX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phosphorylated-SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Dot blot analysis of phosphorylated-Sox2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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