Phospho-MUC1 Antibody (pT1224)

MUC1 is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500, Immunofluorescence: 1:10-1:50

UniProt

P15941

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-MUC1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT1224 of human MUC1.

Clonality

Polyclonal

Isotype

Applications

DB, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-MUC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-MUC1 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-MUC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-MUC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Confocal immunofluorescent analysis of phospho-MUC1 antibody and HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) .