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Phospho-Oct4 Antibody (pS236)

OCT4 encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t (6;22) (p21; q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500

UniProt

Q01860

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-Oct4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS236 of human OCT4.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-Oct4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-Oct4 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-Oct4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-Oct4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Pig

Image Legend

Dot blot analysis of phospho-Oct4 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

Frequently Asked Questions

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