Phospho-IkBa Antibody (pSer32)
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Product Specifications
CAS Number
9007-83-4
Specifications
Dot blot: 1:500
UniProt
P25963
Host
Rabbit
Reactivity
Human
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
DB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-IkBa antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-IkBa antibody is available for research use only.
Storage Conditions
Aliquot the phospho-IkBa antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-IkBa antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Dot blot analysis of phospho-IkBa antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Frequently Asked Questions
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