Phospho-PDX1 Antibody (pT11)

PDX1, Pancreas/duodenum homeobox protein 1, is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this protein are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:100, Dot blot: 1:500

UniProt

P52945

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-PDX1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT11 of human PDX1.

Clonality

Polyclonal

Isotype

Applications

IF, DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-PDX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-PDX1 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-PDX1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-PDX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat

Image Legend

Fluorescent confocal image of SY5Y cells stained with phospho-PDX1 antibody. Alexa Fluor 488 conjugated secondary (green) was used. Note the highly specific localization of the immunosignal to the nucleus.