Phospho-NANOG Antibody (pS285)
NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Product Specifications
CAS Number
9007-83-4
Specifications
Dot blot: 1:500
UniProt
Q9H9S0
Host
Rabbit
Reactivity
Human
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
DB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-NANOG antibody is available for research use only.
Storage Conditions
Aliquot the phospho-NANOG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Dot blot analysis of phospho-NANOG antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Frequently Asked Questions
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