Phospho-ERK1/2 Antibody [p-ERK1/2] (T202/Y204)
MAPK3/ERK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
Product Specifications
CAS Number
9007-83-4
Specifications
Dot blot: 1:500, Western blot: 1:1000
UniProt
P27361
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
DB, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-ERK1/2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-ERK1/2 antibody is available for research use only.
Storage Conditions
Aliquot the phospho-ERK1/2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Drosophila, Rat
Image Legend
Western blot testing of human HeLa and mouse NIH3T3 cells treated with TPA (200nM) and PDGF (100ng/ml) using phospho-ERK1/2 antibody.
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