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Phospho-NSE Antibody (pY236)

NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500

UniProt

P09104

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-NSE antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY236 of human NSE.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-NSE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-NSE antibody is available for research use only.

Storage Conditions

Aliquot the phospho-NSE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-NSE antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat

Image Legend

Dot blot analysis of phospho-NSE antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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