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Phospho-BRAF Antibody (pT400)

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), and a wide range of cancers such as lung cancer, non-Hodgkins lymphoma, and colorectal cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500

UniProt

P15056

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-BRAF antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT400 of human BRAF.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-BRAF antibody is available for research use only.

Storage Conditions

Aliquot the phospho-BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Dot blot analysis of phospho-BRAF antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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