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Phospho-E2F1 Antibody (pS332)

E2F1 is a member of the E2F family of transcription factors. It binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500

UniProt

Q01094

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-E2F1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS332 of human E2F1.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-E2F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-E2F1 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-E2F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-E2F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Dot blot analysis of phospho-E2F1 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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