Phospho-AKT3 Antibody (pS472)
AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1) .
Product Specifications
CAS Number
9007-83-4
Specifications
Dot blot: 1:500
UniProt
Q9Y243
Host
Rabbit
Reactivity
Human
Immunogen
This phospho-AKT3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS472 of human AKT3.
Clonality
Polyclonal
Isotype
Ig
Applications
DB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Store at 4oC for up to one month. For long term, aliquot the phospho-AKT3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-AKT3 antibody is available for research use only.
Storage Conditions
Store at 4°C for up to one month. For long term, aliquot the phospho-AKT3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the phospho-AKT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat
Image Legend
Dot blot analysis of phospho-AKT3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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