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Phospho-AKT3 Antibody (pS472)

AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1) .

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500

UniProt

Q9Y243

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-AKT3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS472 of human AKT3.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the phospho-AKT3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-AKT3 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the phospho-AKT3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the phospho-AKT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat

Image Legend

Dot blot analysis of phospho-AKT3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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