Phospho-AKT Antibody (pS473)
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Product Specifications
CAS Number
9007-83-4
Specifications
Dot blot: 1:500, Western blot: 1:1000
UniProt
P31749
Host
Rabbit
Reactivity
Human
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
WB, DB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-AKT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-AKT antibody is available for research use only.
Storage Conditions
Aliquot the phospho-AKT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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