Phospho-AKT Antibody (pS473)

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500, Western blot: 1:1000

UniProt

P31749

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-AKT antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS473 of human AKT1.

Clonality

Polyclonal

Isotype

Applications

WB, DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-AKT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-AKT antibody is available for research use only.

Storage Conditions

Aliquot the phospho-AKT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of extracts from NIH3T3 cells, untreated or treated with PDGF, using phospho-AKT antibody (left) or nonphos-AKT antibody (right) .