P-Tuberin Antibody (pS939)

Mutations in TSC2/Tuberin lead to tuberous sclerosis complex. The protein is believed to be a tumor suppressor and is able to specifically stimulate the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500, Immunofluorescence: 1:10-1:50

UniProt

P49815

Host

Rabbit

Reactivity

Human

Immunogen

This p-Tuberin antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS939 of human TSC2.

Clonality

Polyclonal

Isotype

Applications

DB, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the p-Tuberin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This p-Tuberin antibody is available for research use only.

Storage Conditions

Aliquot the p-Tuberin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the p-Tuberin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Confocal immunofluorescent analysis of p-Tuberin antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) . DAPI was used as a nuclear counterstain (blue) .