Phospho-Fas Antibody (pY291)

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Fas-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500

UniProt

P25445

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-Fas antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY291 of human Fas.

Clonality

Polyclonal

Isotype

Applications

DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-FAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-Fas antibody is available for research use only.

Storage Conditions

Aliquot the phospho-Fas antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-Fas antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Dot blot analysis of phospho-Fas antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted. P-Pab=phos-Ab; P-Peptide=phos-peptide; NP-Peptide=nonphos-peptide.