P70 S6 Kinase Antibody

RPS6KB1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P23443

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 405-434 from the human protein was used as the immunogen for this p70 S6 Kinase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the p70 S6 Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This p70 S6 Kinase antibody is available for research use only.

Storage Conditions

Aliquot the p70 S6 Kinase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the p70 S6 Kinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Rabbit, Rat

Image Legend

Western blot analysis of p70 S6 Kinase antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (2) . Predicted molecular weight: 60-70 kDa